Foldon unfolding mediates the interconversion between Mpro-C monomer and 3D domain-swapped dimer
- 影响因子：
0.0
- DOI码：
10.1073/pnas.1205241109
- 发表刊物：
Proc Natl Acad Sci U S A
- 摘要：
The C-terminal domain (M(pro)-C) of SARS-CoV main protease adopts two different fold topologies, a monomer and a 3D domain-swapped dimer. Here, we report that M(pro)-C can reversibly interconvert between these two topological states under physiological conditions. Although the swapped α(1)-helix is fully buried inside the protein hydrophobic core, the interconversion of M(pro)-C is carried out without the hydrophobic core being exposed to solvent. The 3D domain swapping of M(pro)-C is activated by an order-to-disorder transition of its C-terminal α(5)-helix foldon. Unfolding of this foldon promotes self-association of M(pro)-C monomers and functions to mediate the 3D domain swapping, without which M(pro)-C can no longer form the domain-swapped dimer. Taken together, we propose that there exists a special dimeric intermediate enabling the protein core to unpack and the α(1)-helices to swap in a hydrophobic environment, which minimizes the energy cost of the 3D domain-swapping process.
- 论文编号：
38
- 卷号：
84
- 页面范围：
9721-8
- 是否译文：
否
- 发表时间：
2012-01-01
- 发布期刊链接：
- 第一作者：
Kang X
- 通讯作者：
Xia Bin
- 全部作者：
Jin C,Zhang S,Zou P,Zhong N
